Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Mesenchymal Stem Cell Extracellular Vesicles Reverse Sugen/Hypoxia Pulmonary Hypertension in Rats

doi: 10.1165/rcmb.2019-0154OC

Figure Lengend Snippet: (A) Immunohistochemical staining of Cd206+ macrophages in lungs of rats treated with normoxia, (B and C) SuHx plus vehicle (B), and SuHx plus MSC-EV (C). Scale bars: 50 μm. (D–F) Average number of Cd206+ per 10× field (D), Cd68+ total rat macrophages per 10× field (E), and ratio of Cd206+/Cd68+ macrophages per 10× lens objective field of view (F) in lung sections from rats treated with Nx, SuHx plus vehicle, or SuHxEV. *P < 0.05, **P < 0.01, and ****P < 0.0001.

Article Snippet: Lungs were kept in 4% paraformaldehyde until embedded in paraffin; sectioned in 5-μm slices; and stained with antibody against rat ACTA2 (ab5694; Abcam) to assess pulmonary vascular remodeling, antibody against rat von Willebrand factor (vWF) (ab6994; Abcam) to assess distal vessel count, and antibodies against rat Cd206 (ab64693; Abcam) or Cd68 (ED1; Bio-Rad Laboratories) for assessment of macrophage recruitment.

Techniques: Immunohistochemical staining, Staining

Journal: Pharmaceutical Biology

Article Title: Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats

doi: 10.1080/13880209.2017.1390589

Figure Lengend Snippet: Immunostaining of ED-1 positive cell in the liver. (A) Normal; (B) control diabetic; (C) diabetic + TGP 50 mg/kg; (D) diabetic + TGP 100 mg/kg; (E) diabetic + TGP 200 mg/kg. Original magnification 100×.

Article Snippet: Mouse anti-macrophage monoclonal (ED-1) antibody was from Abcam Biotechnology (Abcam, Cambridge, UK).

Techniques: Immunostaining

Journal: Investigative ophthalmology & visual science

Article Title: In Vivo Imaging of Fluorescent Probes Linked to Antibodies Against Human and Rat Vascular Endothelial Growth Factor.

doi: 10.1167/iovs.15-18118

Figure Lengend Snippet: FIGURE 5. Choroidal and retinal flatmount staining prepared 7 days following intravitreal injection of bevacizumab-6S-ICC (Cy3-type ICC ¼ indocarbocyanine, red). Staining with CD68 (green, first two rows) showed a colocalization of antibody-conjugates and macrophages, especially within the laser lesions. Staining with Iba1 (green, last two rows) showed also a colocalization with microglial cells. Arrow shows an amoeboid Iba1- positive cell with four small bevacizumab-6S-ICC spots inside (inserted detailed figure).

Article Snippet: Frozen sections of 10 lm were mounted on a glass slide, fixed with 100% methanol and labeled with DAPI (DAPI BioChemica, 40,6-Diamidino-2-phenylindole dihydrochloride; AppliChem GmbH, Darmstadt, Germany), CD68 (mouse anti CD68 IgG1 Clone ED1; Acris Antibodies GmbH, Herford, Germany), and Iba1 (polyclonal anti Iba1, rabbit; Wako Chemicals GmbH, Neuss, Germany).

Techniques: Staining, Injection

Journal: Investigative ophthalmology & visual science

Article Title: In Vivo Imaging of Fluorescent Probes Linked to Antibodies Against Human and Rat Vascular Endothelial Growth Factor.

doi: 10.1167/iovs.15-18118

Figure Lengend Snippet: FIGURE 6. Detection of bevacizumab-6S-ICC (red) in frozen cross sections 7 days following intravitreal injection. In the DAPI stained cross section (A), fluorescent bevacizumab-6S-ICC spots were visible, especially in the area of the laser lesion. Staining for Iba1 shows a moderate colocalization of antibody-conjugates and microglial cells ([B], yellow). A distinct positive double staining with bevacizumab-6S-ICC and CD68 positive macrophages is obvious at the site of CNV (C).

Article Snippet: Frozen sections of 10 lm were mounted on a glass slide, fixed with 100% methanol and labeled with DAPI (DAPI BioChemica, 40,6-Diamidino-2-phenylindole dihydrochloride; AppliChem GmbH, Darmstadt, Germany), CD68 (mouse anti CD68 IgG1 Clone ED1; Acris Antibodies GmbH, Herford, Germany), and Iba1 (polyclonal anti Iba1, rabbit; Wako Chemicals GmbH, Neuss, Germany).

Techniques: Injection, Staining, Double Staining